| Regulation | Description | Series | Episode |
| Away Team Guidelines | In part, forbids the transport of unknown infectious agents onto a starship without first establishing containment and eradication protocols. | VOY | "Macrocosm" |
| Directive 010 | "Before engaging alien species in battle, any and all attempts to make first contact and achieve non-military solutions must be made." | VOY | "In The Flesh" |
| Directive 101 | Assures that an individual accused of a crime has the right to remain silent under questioning. | VOY | "Meld" |
| General Order One (The Prime Directive) | The Prime Directive. Precludes interference in the normal development of a society. The Prime Directive has 47 suborders. When dealing with primitive cultures, the Prime Directive forbids any mention of life on other worlds. | TOS et al. | |
| General Order Seven | Forbids contact with the planet Talos 4. As of 2267, violation of this order was the only death penalty left on the books. | TOS | "The Menagerie" |
| General Order Twelve | Requires adequate precautions to be taken on the approach of any vessel when communications have not been established. | Star Trek II : The Wrath Of Khan | |
| General Order Fifteen | No flag officer may beam into a hazardous area without armed escort. | Star Trek II : The Wrath Of Khan | |
| General Order Twenty Four | A command to destroy the surface of a planet after a specified interval. | TOS | "A Taste Of Armageddon" |
| The Omega Directive | Requires the captain of a starship to notify Starfleet command immediately on detection of an omega molecule, and authorizes the use of any means necessary to destroy it. This directive is unknown to virtually all Starfleet personnel, and over rides the Prime Directive. | VOY | "The Omega Directive" |
| Order 104, Section B | Starfleet order which establishes chain of command. Allows a superior officer to assume command of a Starship when its Captain is absent. | TOS | "The Doomsday Machine" |
| Order 104, Section C | Starfleet order which states the Chief Medical Officer may remove the Captain from command if he considers him unfit for duty. The CMO must produce evidence to support this view. | TOS | "The Doomsday Machine" |
| Regulation 46-A | "If transmissions are being monitored during battle, no uncoded messages on an open channel" | Star Trek II : The Wrath Of Khan | |
| Regulation 42/15 | An engineering procedure related to impulse engines and pressure variations within the impulse reaction chamber tank storage. | TNG | "Relics" |
| Regulation 121, Section A | Starfleet order which states the Chief Medical Officer may remove the Captain from command if he considers him unfit for duty. This order is a replacement or updating of General Order 104, Section C | VOY | "Year Of Hell, Part 2" |
| Regulation 157 Section III Paragraph 18 | Part of the temporal prime directive - "Starfleet officers shall take all necessary precautions to minimize any participation in historical events." | DS9 | "Trials And Tribble-ations" |
| Regulation 476-9 | All away teams must report to the bridge at least once every 24 hours. | VOY | "Once Upon A Time" |
| Security Protocol 28 Subsection D | In the event of a hostile takeover of a Starship, the EMH is to deactivate and await rescue | VOY | "Message In A Bottle" |
| Special Order 66715 | Grants Starfleet Internal Affairs department authority to neutralize security threats to the Federation by whatever means necessary. | DS9 | "Inquisition" |
| Starfleets Temporal Displacement Policy | Part of the temporal prime directive. Prohibits time travelling personnel from interfering in past timelines. | DS9 | "Past Tense, Part 1" |
| Starfleet Engineering Code | Requires a second backup for mission critical components in case the primary backup fails, | DS9 | "Destiny" |
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